selectivity index four breast cancer cell lines Search Results


fucci2 cell reporters mda mb 231 highly metastatic human breast cancer cells  (ATCC)
99
ATCC fucci2 cell reporters mda mb 231 highly metastatic human breast cancer cells
Fig. 3 Mass density distribution between nucleus and cytoplasm is dimensionality-dependent. (a) Epifluorescence images of MDA-MB-231 cells encapsulated in 3D alginate stiff, 3D alginate compliant or on 2D TCP with their corresponding 3D maximum projection tomogram of RI. The epifluorescence images were employed for segmentation of the RI tomogram. Hoechst staining was used for nucleus labelling of cells in 3D alginate groups and the 2D TCP nucleus segmentation was performed based on detectable <t>FUCCI2</t> signal. (b–d) Quantification of refractive index (RI) and dry mass density (r) for nuclear, perinuclear and cytoplasmic regions in 3D alginate stiff, 3D alginate compliant and 2D TCP, respectively. The perinuclear region corresponds to the area in close proximity to the nucleus with 2 mm thickness. Only statistically significant differences were marked. Scale bar equals 10 mm.
Fucci2 Cell Reporters Mda Mb 231 Highly Metastatic Human Breast Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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selectivity index four breast cancer cell lines  (ATCC)
99
ATCC selectivity index four breast cancer cell lines
Fig. 3 Mass density distribution between nucleus and cytoplasm is dimensionality-dependent. (a) Epifluorescence images of MDA-MB-231 cells encapsulated in 3D alginate stiff, 3D alginate compliant or on 2D TCP with their corresponding 3D maximum projection tomogram of RI. The epifluorescence images were employed for segmentation of the RI tomogram. Hoechst staining was used for nucleus labelling of cells in 3D alginate groups and the 2D TCP nucleus segmentation was performed based on detectable <t>FUCCI2</t> signal. (b–d) Quantification of refractive index (RI) and dry mass density (r) for nuclear, perinuclear and cytoplasmic regions in 3D alginate stiff, 3D alginate compliant and 2D TCP, respectively. The perinuclear region corresponds to the area in close proximity to the nucleus with 2 mm thickness. Only statistically significant differences were marked. Scale bar equals 10 mm.
Selectivity Index Four Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
selectivity index four breast cancer cell lines - by Bioz Stars, 2026-06
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mouse breast cancer cell line 4t1  (ATCC)
99
ATCC mouse breast cancer cell line 4t1
Tumor growth process in breast cancer bearing mice. Mice were inoculated with <t>4T1</t> cells and treated with dimethyl sulfoxide (DMSO) of various dose (0.25-1.0 mg/g) or normal saline (NS) for consecutive 10 days intraperitoneal injection Tumors volume was calculated as: L×S 2 ×0.52. (A) Tumor growth curve. The tumor volume growth of experimental groups was inhibited (except for 0.25 mg/g) by a dose and time-dependent way. (B) Tumors were dissected 19 days after inoculation and weighed. Bars represent means±SD. Graph shows tumor weight of experimental groups (except for 0.25 mg/g) was decreased compared with control. * p <0.03 vs. control; † p <0.05 vs. 0.5 mg/g group (n=5).
Mouse Breast Cancer Cell Line 4t1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse breast cancer cell line 4t1 - by Bioz Stars, 2026-06
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Image Search Results


Fig. 3 Mass density distribution between nucleus and cytoplasm is dimensionality-dependent. (a) Epifluorescence images of MDA-MB-231 cells encapsulated in 3D alginate stiff, 3D alginate compliant or on 2D TCP with their corresponding 3D maximum projection tomogram of RI. The epifluorescence images were employed for segmentation of the RI tomogram. Hoechst staining was used for nucleus labelling of cells in 3D alginate groups and the 2D TCP nucleus segmentation was performed based on detectable FUCCI2 signal. (b–d) Quantification of refractive index (RI) and dry mass density (r) for nuclear, perinuclear and cytoplasmic regions in 3D alginate stiff, 3D alginate compliant and 2D TCP, respectively. The perinuclear region corresponds to the area in close proximity to the nucleus with 2 mm thickness. Only statistically significant differences were marked. Scale bar equals 10 mm.

Journal: Soft Matter

Article Title: Optical quantification of intracellular mass density and cell mechanics in 3D mechanical confinement

doi: 10.1039/D0SM01556C

Figure Lengend Snippet: Fig. 3 Mass density distribution between nucleus and cytoplasm is dimensionality-dependent. (a) Epifluorescence images of MDA-MB-231 cells encapsulated in 3D alginate stiff, 3D alginate compliant or on 2D TCP with their corresponding 3D maximum projection tomogram of RI. The epifluorescence images were employed for segmentation of the RI tomogram. Hoechst staining was used for nucleus labelling of cells in 3D alginate groups and the 2D TCP nucleus segmentation was performed based on detectable FUCCI2 signal. (b–d) Quantification of refractive index (RI) and dry mass density (r) for nuclear, perinuclear and cytoplasmic regions in 3D alginate stiff, 3D alginate compliant and 2D TCP, respectively. The perinuclear region corresponds to the area in close proximity to the nucleus with 2 mm thickness. Only statistically significant differences were marked. Scale bar equals 10 mm.

Article Snippet: View Article Online This journal is©The Royal Society of Chemistry 2020 Soft Matter Cell culture and generation of FUCCI2 cell reporters MDA-MB-231 highly metastatic human breast cancer cells (HTB-26; ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (D6046; Sigma-Aldrich) supplemented with 10% v/v fetal bovine serum (Biochrom), and 1% penicillin/streptomycin (Gibco).

Techniques: Staining, Refractive Index

Tumor growth process in breast cancer bearing mice. Mice were inoculated with 4T1 cells and treated with dimethyl sulfoxide (DMSO) of various dose (0.25-1.0 mg/g) or normal saline (NS) for consecutive 10 days intraperitoneal injection Tumors volume was calculated as: L×S 2 ×0.52. (A) Tumor growth curve. The tumor volume growth of experimental groups was inhibited (except for 0.25 mg/g) by a dose and time-dependent way. (B) Tumors were dissected 19 days after inoculation and weighed. Bars represent means±SD. Graph shows tumor weight of experimental groups (except for 0.25 mg/g) was decreased compared with control. * p <0.03 vs. control; † p <0.05 vs. 0.5 mg/g group (n=5).

Journal: Journal of Breast Cancer

Article Title: Dimethyl Sulfoxide Suppresses Mouse 4T1 Breast Cancer Growth by Modulating Tumor-Associated Macrophage Differentiation

doi: 10.4048/jbc.2014.17.1.25

Figure Lengend Snippet: Tumor growth process in breast cancer bearing mice. Mice were inoculated with 4T1 cells and treated with dimethyl sulfoxide (DMSO) of various dose (0.25-1.0 mg/g) or normal saline (NS) for consecutive 10 days intraperitoneal injection Tumors volume was calculated as: L×S 2 ×0.52. (A) Tumor growth curve. The tumor volume growth of experimental groups was inhibited (except for 0.25 mg/g) by a dose and time-dependent way. (B) Tumors were dissected 19 days after inoculation and weighed. Bars represent means±SD. Graph shows tumor weight of experimental groups (except for 0.25 mg/g) was decreased compared with control. * p <0.03 vs. control; † p <0.05 vs. 0.5 mg/g group (n=5).

Article Snippet: Mouse breast cancer cell line 4T1 was purchased from American Type Culture Collection (Manassas, USA).

Techniques: Saline, Injection, Control

Tumor-associated macrophages (TAMs) morphological change and cytotoxicity by dimethyl sulfoxide (DMSO). Peritoneal macrophages were extracted and subcultured in 4T1 tumor cell conditioned medium (TC-medium) containing DMSO of various concentrations. Cell morphology was observed and photographed at 24 and 48 hours. Cytotoxicity tests were performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays at 24 and 48 hours. Interleukin (IL)-12 and IL-10 expression were tested by enzyme-linked immunosorbent assay (ELISA) sandwich method at 48 hours. (A, B) Macrophages were less refractive and branched in the TC-medium compared with control when exposing to DMSO for 24 and 48 hours (original magnification, ×100). The cells which arrows point to indicated the decreasing refractive index and detached macrophages. (C) DMSO exerted cytotoxicity to peritoneal macrophages in TC-medium at 48 hours. Bars represent means±SD. (D) IL-12 expression was increased and IL-10 expression was decreased in ELISA assay compared with control. Bars represented means±SD. * p <0.03 vs. control (n=3); † p <0.01 vs. control (n=5).

Journal: Journal of Breast Cancer

Article Title: Dimethyl Sulfoxide Suppresses Mouse 4T1 Breast Cancer Growth by Modulating Tumor-Associated Macrophage Differentiation

doi: 10.4048/jbc.2014.17.1.25

Figure Lengend Snippet: Tumor-associated macrophages (TAMs) morphological change and cytotoxicity by dimethyl sulfoxide (DMSO). Peritoneal macrophages were extracted and subcultured in 4T1 tumor cell conditioned medium (TC-medium) containing DMSO of various concentrations. Cell morphology was observed and photographed at 24 and 48 hours. Cytotoxicity tests were performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays at 24 and 48 hours. Interleukin (IL)-12 and IL-10 expression were tested by enzyme-linked immunosorbent assay (ELISA) sandwich method at 48 hours. (A, B) Macrophages were less refractive and branched in the TC-medium compared with control when exposing to DMSO for 24 and 48 hours (original magnification, ×100). The cells which arrows point to indicated the decreasing refractive index and detached macrophages. (C) DMSO exerted cytotoxicity to peritoneal macrophages in TC-medium at 48 hours. Bars represent means±SD. (D) IL-12 expression was increased and IL-10 expression was decreased in ELISA assay compared with control. Bars represented means±SD. * p <0.03 vs. control (n=3); † p <0.01 vs. control (n=5).

Article Snippet: Mouse breast cancer cell line 4T1 was purchased from American Type Culture Collection (Manassas, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control, Refractive Index